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Aberrant levels of Cu2+ ions can result in oxidative stress, and has been linked with the development of Indian childhood cirrhosis, prion disease, Menkes disease, Parkinson’s disease and Wilson disease. The upper limit for the concentration of copper in drinking water has been recommended to be 2 ppm by the World Health Organization. A number of Cu2+ -selective chemosensors that employ the chromogenic, fluorogenic, or electrochemical properties of molecules have been reported in the literature. However, these methods may require tedious sample pretreatment and/or multistep synthetic procedures, or they may be limited by an unstable detection signal. Therefore, the development of sensitive and selective sensors for Cu2+ ions is of high interest. The application of transition metal complexes as colorimetric and luminescent probes has recently attracted increasing attention due to their notable advantages. Firstly, the absorptive and emissive behaviour of transition metal complexes can be sensitive to changes in the surrounding environment, allowing changes in analyte concentration to be transduced into an optical response. Secondly, metal complexes can possess significant Stokes shifts, allowing easy distinguishing of excitation and emission light. Third, the relatively long lifetimes of phosphorescent metal complexes compared to organic luminophores can allow interference from scattered light and short-lived background fluorescence to be reduced to a negligible level by use of time-resolved luminescence spectroscopy. Finally, the luminescence quantum yield of transition metal complexes can be enhanced by increased intersystemcrossing rates arising from strong spin-orbit interactions. Among transition metal complexes, octahedral d6 Ir complexes have gained particular interest due to their decent thermal stability, intense luminescence at ambient temperature, and absorption or emission wavelengths across the entire visible light region that can be adjusted by modification of the auxiliary ligands. A few iridium complexes have been developed for Cu2+ detection, such as the phosphorescent cyclometalated iridium complex containing the di -amine copper ion receptor as reported by the group of Lippard, Nam and You, and the phosphorescent cyclometalated iridium complex incorporating 3,9-dithia-6-azaundecane receptor by Hyun and co-workers. In this work, we designed and synthesized a novel cyclometalated iridium complex containing two 2-phenylquinoline C‘N PalGly ligands and a single 5,6-bis -1,10-phenanthroline tetradentate Schiff base receptor, which could function as both a colorimetric and luminescent chemosensor for Cu2+ detection. The synthetic pathway leading to the iridium complex 1 is shown in Figure 2. In our design strategy, the interaction of the Cu2+ ion with the tetradentate Schiff base receptor can induce electron transfer from the metal center to the sa2p ligand, thereby influencing the photophysical behaviour of the iridium complex. Detailed experimental procedures, characterization and photophysical properties of complex 1 are given in the ESI. Transgenic technology enables the introduction of exogenous genes into animal genomes and provides a revolutionary way to achieve elite breeding of livestock. The main applications of transgenic technology in livestock breeding include improving their disease resistance, carcass composition, lactational performance, wool production, growth rate, and reproductive performance, as well as reducing their environmental impact. Our efforts have focused on developing foot-and-mouth disease resistance in dairy cattle using transgenic somatic cell nuclear transfer technology. FMD is a highly contagious vesicular disease of cloven-hoofed animals. Outbreaks of FMD can have severe economic and social consequences that result in the loss of billions of dollars in direct and indirect costs, as well as the slaughter of millions of animals. Current vaccines and disease-control measures to eliminate FMD have many drawbacks. Several new strategies, such as RNA interference, have been developed to control FMD, but few reports have detailed transgenic livestock strategies. Evidence suggests that expression of exogenous IFN-a in livestock confers resistance to FMDV infection. Interferons are widely expressed cytokines that have potent antiviral and growth-inhibitory effects; they are the first line of defense against virus infections. However, several reports indicate that side effects are associated with over-expression of secreted IFN-a in animal models, such as disrupted spermatogenesis in male transgenic mice. In this study, cloned transgenic cattle containing IFN-a were generated to produce FMDV-resistant cattle. A secretory signal sequence of IFN-a was deleted to verify whether intracellular expression of IFN-a has side effects in transgenic cattle. We hypothesized that IFN-a without the secretory signal sequence would elicit the same biologic response as the secreted counterpart, but would not be secreted in transgenic SCNT embryos, would not trigger a signal transduction pathway in transgenic SCNT embryos and between pre-implant transgenic SCNT embryos and endometrial cells, and would have reduced toxicity to neighboring tissues.