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Results: The manual cases occurred over a three and a half year time span after the initial introduction of the testing to the laboratory. There were 1509 cases by manual examination. The results were reported as negative (77.6%), positive (17.1%), unsatisfactory (5.1%), and equivocal (<1%). The image guided system cases occurred over a two year time span. There were 2701 cases by image guided system examination. The results were reported as negative (68.9%), positive (15.2%), unsatisfactory (13.1%) and equivocal (1.5%). A change in the methodology of specimen screening for adequacy prehybridization contributed to an increase in unsatisfactory <a href="">buy GSK3235025 results for the image guided system. Interpretation time commitment varies between methodologies. In general, pathologist manual review requires twenty to thirty minutes and an image guided review requires five to ten minutes. Conclusions: The percentage of positive results showed a good correlation between methods. The difference between negative reporting rates could be attributed to differences in the classification of unsatisfactory specimens. A variety of pre-analytic and pre-hybridization factors can significantly impact reported unsatisfactory rates. Considering the differences in time commitment between the methods, a high volume service could benefit from an image guided system. In our experience, there is good correlation between the manual method and image guided method for the interpretation of UroVysion STA-9090 molecular weight FISH testing of the urinary tract. Introduction: Renal cell carcinomas with translocations resulting in TFEB gene fusion, t(6;11)(p21;q12), are recently discovered translocation-associated renal cell carcinomas of uncertain histogenesis with distinctive clinical and histopathologic features. The predominant cell type in these tumors are clear to oxyphilic, large epithelioid cells that show focal immunohistochemical staining for HMB45 and Melan A and a lack of staining for epithelial markers. Although these tumors share some histopathologic Histamine H2 receptor overlap with Xp11 translocation tumors, unlike Xp11 tumors they occur primarily in pediatric populations, and recent evidence suggests they behave less aggressively. To date, the cytomorphology of these tumors has not been fully characterized. Materials and Methods: We report the case of a 16-year-old female with unremarkable past medical history who presented with hematuria, dysuria, urinary frequency and flank pain. A CT scan for suspected renal calculi (renal stone protocol) demonstrated a 12.5 cm heterogeneously-enhancing renal mass with peripheral calcifications. The mass was displacing the renal hilum but was confined to the kidney. The patient underwent a radical nephrectomy and an intraoperative fine-needle aspiration was performed. Fine-needle aspiration was performed with a 22-guage needle and air-dried (DiffQuik) and alcohol smears (Hematoxylin & Eosin and Papanicolaou) were prepared.